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KMID : 0364820110470010066
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Korean Journal of Microbiology
2011 Volume.47 No. 1 p.66 ~ p.73
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Biodiversity and Isolation of Gut Microbes from Digestive Organs of Harmonia axyridis
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Kim Ki-Kwang
Han Song-Ih
Moon Chung-Won
Yu Yong-Man
Whang Kyung-Sook
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Abstract
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Bacterial density distributions of gut microbes in the digestive organs of Harmonia axyridis collected from three different sources (JK, CK, and CJ) were 6.0×104 CFU/gut under aerobic culture condition and 8.0×106 CFU/gut under anaerobic culture condition. Seven colony types were observed under aerobic condition and three types of similarity were detected under anaerobic condition. In total, 116 strains, including 34 strains under aerobic condition, were isolated from the digestive organs of H. axyridis. Based on the analysis of the 16S rRNA gene sequence, aerobic gut microbes were assigned to the Proteobacteria, Actinobacteria, Firmicutes, and Deinococcus-Thermus. A large number of isolates belonged to the genus Bacillus and Staphylococcus of the Firmicutes commonly found in H. axyridis from different sites. Anaerobic gut microbes were found to be similar according to colony morphological, phylogenetic analysis using ARDRA. Eighty-two anaerobic gut microbes were clustered into 17 different ARDRA types according to HaeIII. Representative
anaerobic gut microbes in each ARDRA group were divided into five species of γ-Proteobacteria based on 16S rRNA gene sequence analysis; Hafnia alvei, Enterobacter ludwigii, Enterobacter kobei, Pseudomonas oryzihabitans and seudomonas koreensis. Phylogenetic analysis indicated that about 70% of the isolates belonged to γ-Proteobacteria, suggesting predominance of gut microbes.
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KEYWORD
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Harmonia axyridis, digestive organ, gut microbes
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